Pyruvate Formate Lyase (pfl)

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immunotherapy

Pyruvate formate lyase (pfl, EC 2.3.1.54) is a bacterial enzyme that converts pyruvate into formate and acetyl-CoA. Because it is the rate-limiting step of microbial formate production, the pfl gene is used as a marker of a microbiome’s formate-producing capacity.

Source papers

  • Exercise-Induced Microbiota Metabolite Enhances CD8 T Cell Antitumor Immunity Promoting Immunotherapy Efficacy Catherine M. Phelps, Nathaniel B. Willis, Tingting Duan, et al., Giorgio Trinchieri, Marlies Meisel · 2025 doi:10.1016/j.cell.2025.06.018