Autophagic Flux

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drug-resistance

Autophagic flux refers to the complete, dynamic throughput of the autophagy pathway — from autophagosome formation, through maturation and fusion with lysosomes, to degradation of their contents — as opposed to a static snapshot of autophagosome number at one point in time. It is commonly assessed via conversion of LC3-I to the lipidated, membrane-associated LC3-II, turnover of the selective autophagy substrate p62/SQSTM1, and phosphorylation of the autophagy-initiating protein Beclin1. Elevated autophagic flux can act as a protective, pro-survival mechanism that helps cancer cells withstand cytotoxic drug treatment.

Source papers

  • The CAGE–miR-181b-5p–S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells Minjeong Yeon, Youngmi Kim, Deepak Pathak, Eunju Kwon, Dong Young Kim, Myeong Seon Jeong, Hyun Suk Jung, Dooil Jeoung · 2021 doi:10.3389/fcell.2021.666387
  • Cancer/testis antigen CAGE mediates osimertinib resistance in non-small cell lung cancer cells and predicts poor prognosis in patients with pulmonary adenocarcinoma Minjeong Yeon, Hankyu Lee, Jeongseon Yeo, Myeong Seon Jeong, Hyun Suk Jung, Hyerim Lee, Kyeonghee Shim, Hyein Jo, Doyong Jeon, Jaemoon Koh, Dooil Jeoung · 2023 doi:10.1038/s41598-023-43124-8
  • HDAC9 and miR-512 Regulate CAGE-Promoted Anti-Cancer Drug Resistance and Cellular Proliferation Minjeong Yeon, Nayeon Kwon, Jaewhoon Jeoung, Dooil Jeoung · 2024 doi:10.3390/cimb46060311
  • Parkin Induces Ubiquitination and Large Extracellular Vesicle Release of HMGB1 to Activate Antitumor Immunity Minjeong Yeon, Michela Perego, Khaled M. Elokely, Magid Abou-Gharbia, Wayne E. Childers, Andrew T. Milcarek, Irene Bertolini, Hsin-Yao Tang, Lucia R. Languino, Gary S. Stein, Prachi N. Ghule, Brad P. Vietje, Douglas T. Taatjes, Dario C. Altieri · 2025 doi:10.1158/0008-5472.CAN-25-0904